AMP Separopore® 4B-CL
AMP Separopore® interacts strongly with NAD+-dependent dehydrogenases and ATP-dependent enzymes. Selective elution with gradients of NAD+ or NADP+ allows the resolution of complex mixtures of dehydrogenase isoenzymes using 5’-AMP Separopore®. The production method involves alkylation of the nucleotide, AMP followed by alkaline rearrangement to yield the corresponding N6-carboxymethyl derivative with subsequent condensation using 1,6-diaminohexane to give N6-[(6-aminohexyl)carbamoylmethyl)].
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.